Document 3250
 DOCN  M94A3250
 TI    Highly LFA-1-expressing U937 subclones showing lower susceptibility to
       HIV-1 can lead to continuously stable virus production after long
       persistent period.
 DT    9412
 AU    Kameoka M; Okada Y; Fujinaga K; Kimura T; Fujii N; Ikuta K; Inst.
       Immunol. Sci., Hokkaido Univ., Sapporo, Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):119 (abstract no. PA0095). Unique
       Identifier : AIDSLINE ICA10/94369325
 AB    OBJECTIVE: Heterogeneous HIV-1 life cycles in monocyte-macrophage
       lineage were examined using U937 subclones for understanding the
       mechanisms of HIV-1 latent or persistent infection. METHODS: A total of
       46 clones (#1-#46) were isolated from U937. The sensitivity to HIV-1
       infection was measured by the appearance of HIV-1 phenotypes.
       Differentiation levels of the clones were examined before and after
       infection. RESULTS: The U937 subclones were classified into three (high,
       middle, and low) types by sensitivity to HIV-1 infection. The high
       sensitivity correlated with the amounts of extra-chromosomal viral
       genome and induction of protein kinase C, but not with the amounts of
       adsorbed and integrated viral genomes. The low-type, but not high-type,
       clones highly expressing LFA-1 antigens were easily differentiated by
       PMA. On the other hand, after infection, the high-type clones were
       easily differentiated, whereas the low-type clones did not, and finally
       became stable, high virus producers after long latent period. DISCUSSION
       AND CONCLUSIONS: Highly LFA-1-expressing clones were differentiated by
       PMA, but failed to be differentiated by HIV-1 infection. The sensitivity
       of these clones to the infection was low, but finally led to stable,
       continuous production of HIV-1. By contrast, the high-type clones
       expressing dim LFA-1 were not differentiated by PMA, but easily
       differentiated by the infection. This difference could be mediated by
       intracellular signaling.
 DE    Cell Differentiation/DRUG EFFECTS/*PHYSIOLOGY  Clone Cells  Comparative
       Study  Human  HIV-1/GENETICS/*PHYSIOLOGY  Lymphocyte Function-Associated
       Antigen-1/*BIOSYNTHESIS  Macrophages  Monocytes  Signal Transduction
       Tetradecanoylphorbol Acetate/PHARMACOLOGY  Tumor Cells, Cultured  Virus
       Integration  *Virus Replication  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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